Probiotic Intake Increases the Expression of Vitellogenin Genes in Laying Hens
2019
Mazanko, Maria S. | Makarenko, Maksim S. | Chistyakov, Vladimir A. | Usatov, Alexander V. | Prazdnova, Evgeniya V. | Bren, Anzhelika B. | Gorlov, Ivan F. | Komarova, Zoya B. | Weeks, Richard | Chikindas, Michael L.
A promising approach for slowing down the rate of reproductive aging is the use of probiotic bacteria as a feed additive. In the current study was investigated the influence of the intake of a potential probiotic on the follicle content and expression of vitellogenin genes (vtg1, vtg2, vtg3) in aged hens. RNA was isolated from liver samples collected from 570-day-old laying hens and gene expression levels were measured using RT-PCR. Bacillus subtilis KATMIRA1933 supplementation had a positive effect on the number of formed follicles in hens and also triggered a significant increase in the relative expression levels of vtg1, vtg2, and vtg3. A Bacillus amyloliquefaciens B-1895 enriched diet or a combination of the two strains had a modest effect on both the number of follicles and the expression of vitellogenin genes. Additionally, the study demonstrates that vitellogenin mRNA expression levels can be considered as a biomarker in a convenient approach for analyzing the hen’s egg-laying ability.
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