Detoxification of corn stover prehydrolyzate by trialkylamine extraction to improve the ethanol production with Pichia stipitis CBS 5776
2011
Zhu, Junjun | Yong, Qiang | Xu, Yong | Yu, Shiyuan
In order to realize the separated ethanol fermentation of glucose and xylose, prehydrolysis of corn stover with sulfuric acid at moderate temperature was applied, while inhibitors were produced inevitably. A complex extraction was adopted to detoxify the prehydrolyzate before fermentation to ethanol with Pichia stipitis CBS 5776. The best proportion of mixed extractant was 30% trialkylamine-50% n-octanol −20% kerosene. Detoxification results indicated that 73.3% of acetic acid, 45.7% of 5-hydroxymethylfurfural and 100% of furfural could be removed. Compared with the undetoxified prehydrolyzate, the fermentability of the detoxified prehydrolyzate was significantly improved. After 48h fermentation of the detoxified prehydrolyzate containing 7.80g/l of glucose and 52.8g/l of xylose, the sugar utilization ratio was 93.2%; the ethanol concentration reached its peak value of 21.8g/l, which was corresponding to 82.3% of the theoretical value.
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