RAPD and AFLP analysis for olive mapping
2002
Rosa, R de la | Martin, A. | Rallo, L. | Angiolillo, A. | Guerrero, C. | Baldoni, L.
Poor information is available on the genetic control of the agronomic traits under selection in olive, but the use of linked markers would permit an early selection of the progenies by the Marker-Assisted Selection (MAS), particularly important for traits related to the mature phase, such as productivity, fruit size and oil quality. We present here the progress on establishing a genetic linkage map for olive using an F1 as segregating population obtained by intervarietal cross between two highly heterozygous cultivars, 'Leccino' and 'Dolce Agogia'. AFLPs and RAPDs were used as dominant markers to locate suitable markers in the map. Only markers heterozygous in one parent and homozygous recessive in the other were useful to produce a map for each parental line. For this reason only bands present in one parent and absent in the other, segregating 1:1 in the F1 progeny, were scored. Data were processed with a PC version of MAPMAKER/EXP 3.0 (Lincoln et al. 1993). For each marker the reversed genotype was also considered because their phase in the gametes was unknown. Linkage values with the highest LOD scores were adopted. A total of 166 AFLP markers were obtained from 15 different primer combinations. 85 markers were scored as heterozygous in 'Dolce Agogia', 81 in 'Leccino'. As far as RAPDs are concerned, 62 primers gave a total of 275 polymorphisms, 151 in 'Leccino' and 124 in 'Dolce Agogia'. 22 linkage groups were obtained for 'Leccino' and 23 for 'Dolce Agogia' with 179 and 167 markers linked respectively. An allele of the Stearoyl-ACP Desaturase gene, involved in the formation of oleic fatty acid in olive oil and previously identified in olive (Baldoni et al., 1996), was located on a linkage group of 'Leccino'.
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