Hill-Activity and P700 Concentration of Chloroplasts Isolated from Radish Seedlings Treated with -Indoleacetic Acid, Kinetin or Gibberellic Acid
2014
Buschmann, Claus | Lichtenthaler, Hartmut K.
The Hill-activity (reduction of DCPIP or methylviologen) and the concentration of P700 were studied in chloroplasts isolated from cotyledons of radish seedlings (R aphanus sativu s L. Saxa Treib), which had been grown with the addition of β-indoleacetic acid (IAA), kinetin, or gibberellic acid.1) The photosynthetic activity of young chloroplasts from 3 day old Raphanus seedlings is very high (c. 180 μmol O₂/mol chlorophyll × h) and decreases continuously thereafter with increasing age. The steady state Hill-activity is readied after 8 to 10 days (values of 55 to 50 μmol 02/mg chlorophyll × h).2) Chloroplasts from plants treated with IAA or kinetin not only exhibit higher plastoquinone levels ¹,², but also a higher P700-content and a higher Hill-activity. The promotion effect is more pronounced with kinetin (+ 36 to 40%) than with IAA (+ 12 to 17%).3) Gibberellic acid has a different effect on composition and activity of chloroplasts. In younger seedlings the Hill-activity appears to be somewhat stimulated, without promotion effect on plasto quinone 2 or P700 concentration. After 10 days GA₃-treated plants show signs of chlorosis combined with a strong decrease in photosynthetic activity.4) The data clearly demonstrate that the composition and activity of the photosynthetic ap paratus are under phytohormone control. IAA and even better kinetin promote the light induced formation of pigment systems and electrontransport chains. GA₃ seems to block the rebuilding of the photosynthetic apparatus under steady state conditions.
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