Analysis of resistance risk and resistance‐related point mutations in Cyt b of QioI fungicide ametoctradin in Phytophthora litchii
2022
Gao, Xuheng | Hu, Shiping | Liu, Zeqi | Zhu, Hongwei | Yang, Jikun | Han, Qingyu | Fu, Yixin | Miao, Jianqiang | Gu, Biao | Liu, Xili
BACKGROUND: Litchi downy blight, caused by Phytophthora litchii, is one of the most important diseases of litchi. Ametoctradin, as the only QioI (quinone inside and outside inhibitor) fungicide, has been registered in China in 2019. However, the ametoctradin‐resistance risk and molecular basis in Phytophthora litchii have not been reported. RESULTS: In this study, the sensitivity profile of 144 Phytophthora litchii strains to ametoctradin was determined, with a mean median effective concentration (EC₅₀) value of 0.1706 ± 0.091 μg mL⁻¹. Nine stable resistant Phytophthora litchii mutants [resistance factor (RF) > 400] were derived from sensitive isolates using fungicide adaption. The compound fitness index of three resistant‐mutants (HN10‐1‐1, HN10‐1‐2 and HN10‐2‐1) was similar or higher than that of their parental isolates in vitro. All these ametoctradin‐resistant mutants were sensitive to metalaxyl, dimethomorph, oxathiapiprolin and cyazofamid. Two point mutations, leading to the S33L and D228N changes in PlCyt b (cytochrome b) were found in ametoctradin‐resistant mutants. Eight ametoctradin‐resistant mutants containing S33L showed increased sensitivity to azoxystrobin and amisulbrom, and one mutant containing D228N exhibited increased sensitivity to cyazofamid. In vitro enzyme activity test showed that ametoctradin could not inhibit the activity of cytochrome bc1 complex with S33L and D228N point mutation. AS‐PCR primers were designed based on the S33L change to detect the ametoctradin‐resistant strains in the future. CONCLUSION: These results suggest that Phytophthora litchii has a medium to high resistance risk to ametoctradin in the laboratory. Two changes, S33L and D228N, in PlCyt b are likely to be associated with the observed ametoctradin resistance. © 2022 Society of Chemical Industry.
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