Transcriptome data on maternal RNA of 24 individual zebrafish eggs from five sibling mothers
2016
Pagano, Johanna F.B. | Rauwerda, Han | de Leeuw, Wim C. | Wackers, P. W. M. | de Jong, Mark | Ensink, Wim | Dekker, Rob | Nehrdich, Ulrike | Spaink, Herman P. | Jonker, Martijs | Breit, Timo M.
Maternal mRNA that is present in the mature oocyte plays an important role in the proper development of the early embryo. To elucidate the role of the maternal transcriptome we recently reported a microarray study on individual zebrafish eggs from five different clutches from sibling mothers and showed differences in maternal RNA abundance between and within clutches, “Mother-specific signature in the maternal transcriptome composition of mature, unfertilized Eggs” [1]. Here we provide in detail the applied preprocessing method as well as the R-code to identify expressed and non-expressed genes in the associated transcriptome dataset. Additionally, we provide a website that allows a researcher to search for the expression of their gene of interest in this experiment.
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