The primary dipole of flipper probes
2021
García-Calvo, José | López-Andarias, Javier | Sakai, Naomi | Matile, Stefan
Despite their growing popularity in biology to image membrane tension, central design principles of flipper probes have never been validated. Here we report that upon deletion of their primary dipole, from electron-poor and electron-rich dithienothiophenes, absorptions blue-shift, lifetimes shorten dramatically, and mechanosensitivity in cells vanishes not partially, but completely.
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