LC–MS and microscale NMR analysis of luciferin-related compounds from the bioluminescent earthworm Fridericia heliota
2011
Marques, Simone M. | Petushkov, Valentin N. | Rodionova, Natalja S. | Esteves da Silva, Joaquim C.G.
This paper presents the main results of RP-HPLC–MS and microscale NMR analysis performed on Accompanying similar to Luciferin (AsLn(x)), compounds present in extracts of the bioluminescent earthworm Fridericia heliota that display similarities with Fridericia’s luciferin, the substrate of the bioluminescent reaction. Three isomers of AsLn were discovered, AsLn(1), AsLn(2) and AsLn(3), all of which present a molecular weight of 529Da. Their UV–Vis absorption spectra show maxima at 235nm for AsLn(1), 238 and 295nm for AsLn(2) and 241 and 295nm for AsLn(3). MSⁿ fragmentation patterns suggest the existence of carboxylic acid and hydroxyl moieties, and possibly chemical groups found in other luciferins like pterin or benzothiazole. The major isomer, AsLn(2), presents an aromatic ring and alkene and alkyl moieties. These luciferin-like compounds can be used as models that could give further insights into the structure of this newly discovered luciferin.
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