Improvement of caper (Capparis spinosa L.) propagation using in vitro culture and gamma irradiation
2011
Al-Safadi, Bassam | Elias, Rana
Some of the factors influencing the propagation of caper (Capparis spinosa L.) plants in vitro and germination of the seed were studied. The number of adventitious shoots emerging from caper stems cultured in vitro increased from 2.2 shoots per explant when the growth medium contained 2mg/L of gibberellic acid (GA3) to 5.5 when the growth medium contained 2mg/L zeatin riboside (ZR) and 1mg/L naphthalene acetic acid (NAA). The best medium for callus formation from leaf and stem parts contained the growth regulators 1mg/L 6-benzylaminopurine (BAP) and 0.1mg/L NAA and the best medium for plant regeneration contained 1mg/L kinetin and 0.1mg/L indole-3-acetic acid (IAA). The effect of gamma irradiation on the growth of caper shoots in vitro was also studied. A 10Gy dose of gamma irradiation stimulated growth of shoots up to 200% and increased shoot rooting percentage from 75 to 100%. Methods of scratching the seed coat with iron particles and treating the whole seeds with concentrated H₂SO₄, ultrasonic waves and gamma rays were employed for breaking the seed dormancy. Treating the seed with H₂SO₄ for 20min along with scratching was very effective in stimulating germination (46% as compared with 0% for the control). Irradiating caper seeds with 100Gray (Gy) dose of gamma rays led to 50% seed germination in vitro and 70% on peatmoss one month after culturing.
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