Gastrothylax crumenifer: ultrastructure and histopathology study of in vitro trematodicidal effect of Microlepia speluncae (L.) Moore
2018
Kalpana Devi, R. | Vasantha, S. | Panneerselvam, A. | Rajesh, N. V. | Jeyathilakan, N. | Venkataramanan, R.
An in vitro incubation study was carried out to determine the efficacy of Microlepia speluncae (MS) fronds’ extract on the worm motility and viability, morphology and histopathological changes in/of Gastrothylax crumenifer. The methanolic extract of MS fronds was used for in vitro study at 1, 2, 3, 4 and 5 mg/ml concentration as test extracts. Oxyclozanide (OXY) at 1% (250 mg/25 ml) as standard control and Hedon–Fleig (H–F) salt solution as negative control. The solutions (25 ml), with 25 amphistomes each, distributed to 7 petriplates (90 mm diameter) and placed in an incubator with 5% CO₂ at 37°C. The motility of control and treated flukes was observed at regular time intervals, 0, 10, 15, 30 and 60 min respectively with specific score 3, 2, 1, 0 for motility. Relative Motility assay and LC₅₀ were determined by probit regression analysis. In vitro incubation study revealed death of all trematodes, lethal at 10 min incubation time at 5 mg/ml concentration, indicated RM value is 0, very much effective than Oxyclozanide, RM value was 0. 05. The LC₅₀ was determined as 3.666. The worms subjected to in vitro trials were studied under Scanning Electron Microscope and Light Microscope for significant morphological changes.
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