VNTR polymorphism in the ITS1 region (rDNA) in isolates of a Nodulisporium species
2002
Platas, Gonzalo | Polishook, Jon D. | Peláez, Fernando
Variable Number of Tandem Repeats (VNTR) polymorphism has been identified in the ITS1 region of the rDNA in 13 strains belonging to a species of Nodulisporium producing the novel indole diterpene nodulisporic acid. The number of tandem repeats found in these isolates varies from 2, 4 or 5 repetitions, and produces three length morphs in the ITS1 rDNA, spanning a length difference of 217, 308 and 352 nucleotides. Repeated units are rather variable, and they encompass three tandem repeated motifs of 11 nucleotides. In all the individuals, the variants are positioned in the same physical order, following the distribution of the most parsimonious tree. This suggests that this sequence have been enlarged by slipped-strand mispairing occurrences.
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