Ozone effects on soluble protein content of Acer negundo, Quercus robur and Platanus spp. pollen
2013
Ribeiro, H. | Duque, L. | Sousa, R. | Abreu, I.
In this study, the effect of three different ozone (O₃) levels on soluble protein content of Acer negundo, Quercus robur and Platanus spp. pollen was investigated. Pollen was directly collected from each plant and exposed to O₃ values below, equal and four times the limit for human health protection in an environmental chamber system. With this chamber, it was possible to control and reproduce the O₃ levels between different experiments. Total protein content was determined colorimetrically with Coomassie protein assay reagent (Pierce) by the Bradford method. Results showed that O₃ has significant effects on pollen causing a decrease in soluble proteins that seem to be dependent on species and gas concentration. Also, the existence of reproducibility in the effects of O₃, within the same concentration, on the protein content of pollen was verified.
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