Antioxidant Activity and Inhibition of Lipid Peroxidation in Germinating Seeds of Transgenic Soybean Expressing OsHGGT
2011
Kim, Yul Ho | Lee, Yu Young | Kim, Yŏng-ho | Choi, Man Soo | Chŏng, Kwang-ho | Lee, Seuk Ki | Seo, Min Jung | Yun, Hong Tai | Lee, Choon Ki | Kim, Wook Han | Lee, Sang-Chul | Park, Soon Ki | Park, Hyang Mi
Tocochromanols are potent lipid-soluble antioxidants and essential nutrients for human health. Genetic engineering techniques were used to develop soybeans with enhanced vitamin E levels, including tocotrienols, which are not found in soybean. The gene encoding rice homogentisate geranylgeranyl transferase (HGGT) was overexpressed in soybeans using seed-specific and constitutive promoters. The association between abundance of vitamin E isomers and antioxidant activity was investigated during seed germination. With the exception of β-tocotrienol, all vitamin E isomers were detected in germinating seeds expressing OsHGGT. The antioxidant properties of germinating seed extracts were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radicals and lipid peroxidation (TBARS). Compared with intact wild-type seeds, transgenic seeds showed increases in radical scavenging of 5.4−17 and 23.2−35.3% in the DPPH and ABTS assays, respectively. Furthermore, the lipid peroxidation levels were 2.0−4.5-fold lower in germinating seeds from transgenic lines than in wild-type seeds. Therefore, it appears that the antioxidant potential of transgenic oil-producing plants such as soybean, sunflower, and corn may be enhanced by overexpressing OsHGGT during seed germination.
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