A novel HPLC-DAD method with dilute-and-shoot sample preparation technique for the determination of buprofezin, dinobuton and chlorothalonil in food, environmental and biological samples
2021
Gebrehiwot, Weldejeworgis Hndeya | Erkmen, Cem | Uslu, Bengi
The development of agriculture is closely related to the use and application of pesticides in order to improve products. Excessive use of pesticides is indeed very dangerous to essential pests and it has a potential negative effect over human health, pollution of soil and waters. For these reasons, in this study, a dilute-and-shoot sample preparation procedure for simultaneous determination of three pesticides (buprofezin, dinobuton and chlorothalonil) was developed and validated for the first time combined with high-performance liquid chromatography–diode-array detector method in urine, serum, tomato, soil, commercial dosage form and water samples. Also, the degradation and behaviour of these pesticides were investigated under different conditions in the environment. HPLC analysis was performed with Kinetex C18 (150 mm × 4.6 mm; 5 μm) column with 1 mL min⁻¹ flow rate of mobile phase which consisted of acetonitrile/buffer in the ratio of 75:25 (v/v) at 25.0°C. Calibration graphs showed a good linearity with a coefficient of determination (R ²) of at least 0.999 for all analytes. Intraday and interday precisions (expressed as RSD%) were lower than 1.66% for all analytes. The developed method was demonstrated to be sensitive, simple, robust and rapid for the simultaneously determination of analytes in different samples providing recoveries in the range between 92.3% and 109.74%.
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