Identification and physical mapping of three Haynaldia villosa chromosme-6V deletion lines
1998
Qi, L.L. | Wang, S.L. | Chen, P.D. | Liu, D.J. | Gill, B.S.
Three deletion lines (del6V#2S-1, del6V# 2L-1, and del6V#2L-2) of Haynaldia villosa chromosome 6V added to wheat were identified by C-banding and characterized by RFLP analyses. The breakpoints were located at fraction lengths (FL) 0.58 in del6V#2S-1 in the short arm, and FL 0.66 in del6V#2L-l and FL 0.64 in del6V#2L-2 in the long arm. Thirty-one Triticeae homoeologous group-6 DNA probes were used to map RFLP loci in the deletion lines and the wheat-H. villosa disomic substitution (DS) line 6V#2(6A). Nine probes failed to detect polymorphism between Chinese Spring and DS6V#2(6A). Ten of sixteen polymorphic short-arm loci were not detected in del6V#2S-1. Thus, the loci are located in the deleted distal chromosome region. Six RFLP markers were mapped in the proximal 58% of 6VS. Of 20 DNA markers specific for 6VL, six mapped in the distal 36% of the long arm, and nine mapped in the proximal 64% of 6VL. The breakpoint of the short arm of 6V#2 occurs between Xpsr106 and Xcdo270, and that of the long arm between Xpsr915 and Xmwg934. The powdery mildew resistance gene Pm21 is located on the short arm of chromosome 6V#2. Pm21 is present in del6V#2S-1, and can be further mapped in the proximal 58% of 6V#2S.
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