Effects of chloride ion on acutumine and dechloroacutumine production by Menispermum dauricum root culture
1999
Babiker, H.A.A. | Sugimoto, Y. | Saisho, T. | Inanaga, S.
The effects of chloride ion on the production of acutumine and dechloroacutumine, by Menispermum dauricum root culture, were studied. The chloride ion content in the medium plays a key role in the production of both alkaloids. A low chloride medium promoted production of dechloroacutumine and suppressed that of acutumine. Production of the two alkaloids during the 60 day culture period was closely associated with root biomass. Both alkaloids accumulated in the roots and a relatively small portion was exuded into the medium. The intact plant produced very low amounts of both alkaloids. On the average, cultured roots contained 22- and 75-fold more acutumine and dechloroacutumine, respectively, than intact plants.
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