Characterization of Marinilongibacter aquaticus gen. nov., sp. nov., a unique marine bacterium harboring four CRISPR-Cas systems in the phylum Bacteroidota
2022
Zhang, Dao-Feng | Yao, Yu-Fang | Xue, Hua-Peng | Fu, Zi-Yue | Zhang, Xiao-Mei | Shao, Zongze
A novel bacterium, designated YYF0007ᵀ, was isolated from an agar-degrading co-culture. The strain was found harboring four CRISPR-Cas systems of two classes in the chromosome and subsequently subjected to a study on polyphasic taxonomy. Pairwise analyses of the 16S rRNA gene sequences indicated that strain YYF0007ᵀ had highest 16S rRNA gene sequence similarity (92.2%) to Jiulongibacter sediminis JN-14-9ᵀ. The phylogenomic trees based on the 16S rRNA gene and 269 single-copy orthologous gene clusters (OCs) indicated that strain YYF0007ᵀ should be recognized as a novel genus of the family Spirosomaceae. The cells were Gramstain-negative, nonmotile, strictly aerobic, and straight long rods with no flagellum. Optimum growth occurred at 28°C and pH 7.0 with the presence of NaCl concentration 1.0–3.0% (w/v). The strain showed oxidase and catalase activities. The major fatty acids were C₁₆:₁ω5c, iso-C₁₅:₀ and summed feature 3 (C₁₆:₁ω7c and/or C₁₆:₁ω6c). The predominant isoprenoid quinone was MK-7. The complete genome size was 4.64 Mb with a DNA G + C content of 44.4%. Further typing of CRISPR-Cas systems in the family Spirosomaceae and the phylum Bacteroidota indicated that it was remarkable for strain YYF0007ᵀ featured by such a set of CRISPR-Cas systems. This trait highlights the applications of strain YYF-0007ᵀ in studies on the evolutionary dynamics and bacterial autoimmunity of CRISPR-Cas system as a potential model. The name Marinilongibacter aquaticus gen. nov., sp. nov. is proposed, and the type strain is YYF0007ᵀ (= MCCC 1K06017ᵀ = GDMCC 1.2428ᵀ = JCM 34683ᵀ).
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