Dynamics of ATP and movement in Eurasian perch (Perca fluviatilis L.) sperm in conditions of decreasing osmolality
2009
Boryshpolets, S. | Dzyuba, B. | Stejskal, V. | Linhart, O.
Repetitive activation of perch (Perca fluviatilis L.) sperm motility was investigated in this study. The first phase of sperm motility activation was initiated by dilution in a 260mM glucose solution (75% motility). The second phase of motility was achieved by adding water to previously activated sperm, so that the glucose concentration dropped to 220mM (24% motility). Finally, the third phase was obtained by further addition of water (down to 90mM glucose) to the activated sperm suspension (15% motility). Parallel measurements of sperm ATP content were also made. The median value for nonactivated sperm was 43.9 nmol ATP/10⁹ spermatozoa. The ATP concentration decreased significantly from 35 to 7 nmol ATP/10⁹ spermatozoa after successive activations of motility in the above glucose solutions. Sperm velocity ranged in value from 25 to 330μm/sec at 10sec postactivation, from 10 to 290μm/sec at 30sec, and from 0 to 200μm/sec at 45sec. A model postulating several classes in the population of spermatozoa is developed, tentatively accounting for such successive activation. Possible further application of multiple sperm activation is discussed.
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