Patterns of molecular evolution of microsatellite loci in pepper (Capsicum spp.) revealed by allele sequencing
2009
Stágel, Anikó | Gyurján, István | Sasvári, Zsuzsanna | Lanteri, Sergio | Ganal, Martin | Nagy, István
A total of 28 fragments from five microsatellite loci containing simple to highly complex repeat motifs were amplified from a number of Capsicum accessions and subsequently cloned and sequenced. In addition to variation in the number of repeats, allelic differences were found, especially in the more complex microsatellites, that are the consequences of various mutations including base substitutions, insertions/deletions, and rearrangements resulting in 21 distinct alleles. A number of the observed allelic differences were in part not revealed by the use of standard DNA-sizing technologies. The possible mutation and evolution mechanisms and the consequences of using complex and/or compound microsatellite markers for evolutionary, diversity, and taxonomical studies are discussed.
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