Separation and quantitative determination of grape and wine procyanidins by high performance reversed phase liquid chromatography
1990
Silva, J.M.R. da | Rosec, J.P. | Bourzeix, M. | Heredia, N.
A method is described for the separation and quantitative analysis of procyanidins in grape and wine. On a polyamide chromatographic column an initial elution with water at pH 7.0 enabled elimination of interfering phenolic acids. A second elution with acetonitrile/water (30:70 v) was performed to extract catechins. A third elution with acetone/water (75:25 v) was carried out to obtain procyanidins. The quantitative determination of procyanidins was done by high performance reversed phase liquid chromatography. The HPLC analysis was brought down to approximately 55 min. The method is accurate arid reproducible and has an acceptable extraction efficiency.
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