Decomposition Products of Carbon-14 Labelled Rye Tissue in a Peat Profile
1966
Chahal, K. S. | Mortensen, J. L. | Himes, F. L.
After rye (Secale Ceerale) tissue, labelled with C¹⁴, had been incubated with a peat soil, the soil was extracted with various solvents. One fractionation procedure was the proximate analysis method according to Waksman and Stevens (15). The other procedure was a comparison of the components extracted by 0.1m Na₄P₂O₇, 0.5n NaOH, and 6n HCl. In the proximate analysis, the distribution of total carbon in benzene-methano (10:1), 2% HCl, 80% H₂SO₄, and insoluble portion was 1.9, 30.8, 17.2, and 50.1%, respectively. The respective distribution of C-14 was 40.3, 20.5, 13.4, and 25.8%. The fraction containing the smallest proportion of carbon had the largest proportion of C-14. The 6N HCl extract was separated into more discrete fractions by gel infiltration than the extracts of 0.1M Na₄P₂O₇ and 0.5n NaOH. However, the NaOH removed a greater proportion of the radioactive materials.
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