Do camels (Camelus dromedarius) play an epidemiological role in the spread of Shiga Toxin producing Escherichia coli (STEC) infection
2008
El-Sayed, A | Ahmed, S | Awad, W
In the present work, faecal and serum samples from 400 camels were investigated for the presence of Shiga Toxin producing E.coli (STEC) and Anti-Shiga Toxin (Anti-Stx) antibodies, respectively. The used samples were obtained from adult male camels of five east African countries (Egypt, Somalia, Djibouti, Kenya and Sudan) between the years 2002-2004. One E.coli isolate per camel was randomly selected to be cultured on Gassner, Chromocult and sorbit agar for the detection of O157:H7 strains. In the same time, a Stx-specific PCR screening was performed for the isolates using the shiga toxin specific primers Mk1-Mk2. Vero cells were also used for shiga toxin neutralization assay. None of the investigated isolates reacted positively with the Stx-specific primers. Also, none of the studied sera could neutralize the Stx on tissue culture. The obtained results indicate that camels do not play any significant epidemiological role in STEC infection and transmission. The possible reasons for the absence of STEC in the investigated samples are discussed in brief.
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