The Inhibition Analysis of Two Heavy Metal ATPase Genes (NtHMA3a and NtHMA3b) in Nicotiana tabacum
2015
Chang, S. | Shu, H.
Previous studies suggested that plants detoxified mercury and cadmium through similar mechanisms. A heavy metal ATPase (adenosine triphosphatase) gene, HMA3, plays a key role in the plant's cadmium detoxification. To prove whether HMA3 also participates in mercury detoxification in plants, an experiment was designed to inhibit the expressions of HMA3 genes (NtHMA3a and NtHMA3b) in tobacco plants. Results showed that plants’ tolerance to mercury ions had not changed after the expressions of NtHMA3a and NtHMA3b were inhibited. When mercury content was measured from the whole seedlings, no differences had been observed among wild-type, NtHMA3a-NtHMA3b-RNAi, and the empty-vector transgenic plants. HMA3 was not the key gene responsible for plants’ mercury ion uptake from soil. Although the mercury content in the root was higher than that in the shoot for each seedling, in each treatment, neither in shoots nor in roots were statistical differences in mercury content found among NtHMA3a-NtHMA3b-RNAi, empty-vector transgenic, and wild-type plants. After the expressions of NtHMA3a and NtHMA3b were inhibited, the movement of mercury ions from root to shoot had not been affected. HMA3 was not the key gene responsible for mercury ion transportation from root to shoot. When mercury content was measured from the whole seedling, no significant difference had been found among wild-type, NtHMA3a-NtHMA3b-RNAi, and the empty-vector transgenic plants. For mercury ion translocation across tonoplast, the main pathway might not be HMA3, but ABC (ATP-binding cassette) transporters.
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