Butanol production from hemicellulosic hydrolysate of corn fiber by a Clostridium beijerinckii mutant with high inhibitor-tolerance
2013
Guo, Ting | He, Ai-yong | Du, Teng-fei | Zhu, Da-wei | Liang, Da-feng | Jiang, Min | Wei, Ping | Ouyang, Ping-kai
A Clostridium beijerinckii mutant RT66 with considerable inhibitor-tolerance obtained by continuous culture was used for butanol production from non-detoxified hemicellulosic hydrolysate of corn fiber treated with dilute sulfuric acid (SAHHC). In fed-batch fermentation, 1.8L of diluted SAHHC containing 10g/L of reducing sugar was provided during the acidogenic phase and 0.2L of concentrated SAHHC containing 300g/L of reducing sugar was provided during the solventogenic phase. The mutant produced a total amount of solvents of 12.9g/L, which consisted of 3.1g/L of acetone, 9.3g/L of butanol and 0.5g/L of ethanol. A solvent yield of 0.35g/g sugar and a productivity of 0.18g/Lh in 72h were achieved. The remarkable inhibitor-tolerance of C. beijerinckii RT66 demonstrates that this may be an excellent strain for butanol production from ligocellulosic materials.
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