1-Naphthyl acetate-dependent medium acidification by Zea mays L. coleoptile segments
1991
Salguero, J. | Calatayud, A. | Gonzalez-Daros, F. | Valle-Tascon, S. del
Zea mays L. cv INRA 5a coleoptile segments acidify the incubation medium on the addition of 1-naphthyl acetate (1-NA). The buffering capacity of the bathing solution increases during 1-NA stimulated medium acidification. The solution bathing the 1-NA treated coleoptile segment was analyzed by high performance liquid chromatography. A considerable amount of acetic acid was detected in the bathing solution used to measure 1-Na-dependent medium acidification. For the first time, the data demonstrate directly the release of acetic acid from 1-NA. The extent of medium acidification was proportional to 1-NA concentration. Simultaneous measurement of medium acidification and acetate content upon addition of 1-NA showed that both processes were temporally correlated. The stoichiometry of proton equivalents to acetate ion was 0.966. Addition of 50 micromolar N,N'-dicyclohexylcarbodiimide had little effect on 1-NA-dependent medium acidification. The results indicate that 1-NA is hydrolyzed in the extracellular space of coleoptile cells.
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