Sulfated polysaccharide from the red algae Gelidiella acerosa: Anticoagulant, antiplatelet and antithrombotic effects
2020
Chagas, Francisco Diêgo da Silva | Lima, Glauber Cruz | dos Santos, Valesca Ingrid Nobre | Costa, Luís Eduardo Castanheira | de Sousa, Willer Malta | Sombra, Venícios Gonçalves | de Araújo, Diego Freitas | Barros, Francisco Clark Nogueira | Marinho-Soriano, Eliane | de Andrade Feitosa, Judith Pessoa | de Paula, Regina Célia Monteiro | Pereira, Maria Gonçalves | Freitas, Ana Lúcia Ponte
A sulfated polysaccharide from the red algae Gelidiella acerosa (GaSP) was obtained through enzymatic extraction and subjected to chemical characterization by HPSEC, elemental microanalysis, FT-IR and NMR spectroscopies. The GaSP anticoagulant activity was investigated through APTT and PT tests and platelet aggregation assessed by turbidimetry. The antithrombotic and hemorrhagic activities were evaluated by venous thrombosis and hemorrhagic tendency models, respectively. FT-IR and NMR demonstrated that GaSP is a sulfated agaran. HPSEC and elemental microanalysis revealed a peak molar mass of 284.8 kDa and a degree of sulfation of 0.63, respectively. This molecule prolonged the coagulation time in 2.1 times and inhibited the platelet aggregation by 45%. Furthermore, it showed significant dose-dependent antithrombotic effect of 40%, 64% and 80% at 0.1, 0.5 and 1 mg/kg, respectively, without hemorrhage. These results suggest that GaSP has promising antithrombotic.
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