The cloning and characterization of a DEAD-Box RNA helicase from stress-responsive wheat
2014
Zhang, Xin-mei | Zhao, Xiao-qiong | Feng, Chuan-xin | Liu, Na | Feng, Hao | Wang, Xiao-jie | Mu, Xiao-qian | Huang, Lili | Kang, Zhen-sheng
DEAD-box RNA helicases play important roles in all types of processes in RNA metabolism. This report characterizes a stress-responsive transcript termed TaRH1 (Triticum aestivum RNA helicase) that encodes a putative ATP-dependent RNA helicase and is a member of the DEAD-box family. Quantitative real-time PCR (qRT-PCR) analysis indicated that the TaRH1 gene was differentially expressed under both biotic and several abiotic stresses. The characteristics are given of the TaRH1-catalysed unwinding of duplex RNA following TaRH1 expression in Escherichia coli BL 21. These results suggest that the TaRH1 gene may participate in the plant stress response.
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