Optimization of culturing conditions for production of somatic embryos and lignins of Schisandra chinensis (Turcz.) Baill
2018
Sun, Dan | Wang, Zhenxing | Yu, Yunfei | Li, Changyu | Qin, Hongyan | Xu, Peilei | Zhao, Ying | Liu, Yingxue | Yang, Yiming | Fan, Shutian | Ai, Jun
Schisandra chinensis (Turcz.) Baill. is a valuable medicinal plant species increasingly used in phytotherapy worldwide. This study systematically detected the lignin content and production during somatic embryogenesis of S. chinensis. The effect of various culture parameters on biomass accumulation and lignin production were also examined to optimize the accumulation of lignins in SEs in bioreactors, including the culture method, inoculum density, aeration volume and photoperiod. An inoculum density of 20 g L⁻ ¹ embryogenic calli enhanced production of lignin, while 30 g L⁻ ¹ embryogenic calli increased the biomass of somatic embryos. During somatic embryo induction, an aeration volume of 0.2 vvm and photoperiod of 16 h day⁻ ¹ were found to be optimal for biomass accumulation and lignin production. An approximately threefold increase in the biomass production rate and a fourfold increase in the total lignin production rate in SEs were achieved in bioreactors than on solid medium. The present study indicated, therefore, that the culturing of S. chinensis somatic embryos in bioreactors is an effective method for the industrialized production of lignin in vitro.
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