Analysis of ABC genes during melon ripening
2017
Corbacho, J. | Gomez-Jimenez, M. C.
The ATP-binding cassette (ABC) transporter superfamily is the largest transporter gene family responsible for transporting specific molecules across lipid membranes in all living organisms. Although the superfamily of ABC proteins plays key roles in the physiology and development of plants, the functions of members of this family mostly remain to be clarified, especially in crop plants. Thus, analysis of this family in melon (Cucumis melo L.) will be helpful in the design of strategies for functional analysis. Here, we identified 13 putative ABC genes from Charentais melon fruit and analyzed their phylogenetic relationships. Gene expression analysis revealed that they are differentially regulated dependent on tissue and developmental stage. Moreover, we have investigated their temporal expression patterns during melon ripening using quantitative real-time PCR. Our results indicate that six ABC genes (CmABCB25, CmABCF1, CmABCF4, CmABCE2, CmABCC4 and CmABCI17) were up-regulated during melon ripening. Of these, the expression of two ABC genes, CmABCC4 and CmABCI17, was reduced in ethylene-suppressed antisense ACC oxidase fruit, suggesting that the expression of these genes was potentially regulated by ethylene. Our analysis provides novel information for the ABC genes during ripening and lays a foundation for future analysis of their hormonal regulation.
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