Bioremediation of Lindane Contaminated Soil by Pleurotus ostreatus in Non Sterile Conditions Using Multilevel Factorial Design

Rigas, F.; Papadopoulou, K.; Philippoussis, A.; Papadopoulou, M.; Chatzipavlidis, J.

Bioremediation of Lindane Contaminated Soil by Pleurotus ostreatus in Non Sterile Conditions Using Multilevel Factorial Design

2009

Rigas, F. | Papadopoulou, K. | Philippoussis, A. | Papadopoulou, M. | Chatzipavlidis, J.

A commercial strain of the fungus Pleurotus ostreatus was used to mediate the degradation of lindane, by landfarming technology during a 4 weeks statistical experiment. The Multilevel Factorial Design was used with two design factors, namely, straw content X ₁ (%) and lindane content X ₂ (ppm). The optimization parameters (responses) investigated were: biodegradation rate Y ₁ (μg d⁻¹), biomass growth rate Y ₂ (mg d⁻¹), biodegradation/biomass Y ₃ (μg mg⁻¹), total organic carbon Y ₄ (%), total organic nitrogen Y ₅ (%) and total organic carbon/total organic nitrogen Y ₆. The optima of the adequate models obtained for the period of 2 and 4-weeks were found. An overall kinetic study, conducted in this work with the aid of experimental design, determined the optimum (maximum) specific lindane degradation rate to be 0.16 g kg⁻¹ month⁻¹.

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