Calcium and calcium receptor CAS promote Arabidopsis thaliana de‐etiolation
2012
Huang, Shan‐Shan | Chen, Juan | Dong, Xue‐Jun | Patton, Janet | Pei, Zhen‐Ming | Zheng, Hai‐Lei
As a second messenger, the free cytosolic calcium ion (Ca2+) plays important roles in many biochemical and physiological processes including photosynthesis in plants. In this study, we investigated morphological changes, chlorophyll accumulation and chloroplast development during early photomorphogenesis in etiolated seedlings of both Arabidopsis thaliana wild type (WT) and those with the antisense of CAS, a calcium sensor (CASas). Seedlings were grown at high, medium and low Ca2+ concentrations to identify the roles of Ca2+ and CAS in de‐etiolation and chloroplast development. The results demonstrated that Ca2+ and CAS are correlated with de‐etiolation of A. thaliana after light exposure. High Ca2+ significantly increased chlorophyll content and improved chloroplast development in both A. thaliana WT and CASas etiolated seedlings during de‐etiolation. The analysis by western blot and real‐time fluorescent quantitative polymerase chain reaction indicated that the expression levels of CAS mRNA and protein were upregulated by white light and external Ca2+ significantly. Etiolated CASas plants showed much lower chlorophyll content and delay of chloroplast development as compared with WT plants, indicating that CAS functions in de‐etiolation. All together, we concluded that the de‐etiolation in A. thaliana was promoted by the high Ca2+ concentration and CAS expression to a certain extent.
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