Different micro-aeration rates facilitate production of different end-products from source-diverted blackwater

The effects of micro-aeration on the performance of anaerobic sequencing batch reactors (ASBR) for blackwater treatment were investigated in this study. Different micro-aeration rates, 0, 5, 10, 50, and 150 mg O₂/L-reactor/cycle, and their effect on the hydrolysis, acidogenesis, and methanogenesis of blackwater were evaluated and compared at ambient temperature. Source-diverted blackwater (toilet water) contains high organic contents which can be recovered as biogas. Previous studies have found that anaerobic digestion of blackwater without micro-aeration can only recover upwards of less than 40% of chemical oxygen demand (COD) to methane at room temperature due to the low hydrolysis rate of biomass content in blackwater. This study achieved increases in blackwater hydrolysis (from 34.7% to 48.7%) and methane production (from 39.6% to 50.7%) with controlled micro-aeration (5 mg O₂/L-reactor/cycle). The microbial analysis results showed that hydrolytic/fermentative bacteria and acetoclastic methanogens (e.g. Methanosaeta) were in higher abundances in low-dose micro-aeration reactors (5 and 10 mg O₂/L-reactor/cycle), which facilitated syntrophic interactions between microorganisms. The relative abundance of oxygen-tolerant methanogen such as Methanosarcina greatly increased (from 1.5% to 11.4%) after oxygen injection. High oxygen dosages (50 and 150 mg O₂/L-reactor/cycle) led to reduced methane production and higher accumulation of volatile fatty acids, largely due to the oxygen inhibition on methanogens and degradation of organic matters by aerobic growth and respiration, as indicated by the predicted metagenome functions. By combining reactor performance results and microbial community analyses, this study demonstrated that low-dose micro-aeration improves blackwater biomethane recovery by enhancing hydrolysis efficiency and promoting the development of a functional microbial population, while medium to high-dose micro-aeration reduced the activities of certain anaerobes. It was also observed that medium-dose micro-aeration maximizes VFA accumulation, which may be used in two-stage anaerobic digesters.