Physical Mapping of 18S rRNA Gene in Green Mussel Perna viridis – An Indication of Higher Major rRNA Gene Clusters
2022
Muhammed Zafar Iqbal, A. N. | Khan, Mohd Sajid | Navalgund, M. A. | Goswami, U.
The family Mytilidae generally shows a genus-specific diploid number (2n) of chromosomes; however, one species from each genus, with the exception of Mytilus, is known to have a variable diploid number. Perna viridis is a cytogenetically significant species because it has a diploid chromosome (2n) number of 30, whereas the rest of species of genera Perna and Mytilus have a diploid number of 28. In the present study, we amplified the partial sequence of the 18S rRNA gene of Perna viridis, and the fluorescence (DIG-dUTP) labeled probe was hybridized to the interphase and metaphase chromosomes. The results localize the 18S rRNA gene clusters on the short arms of four chromosome pairs 1, 3, 7, and 11. This coincides with the position of the NORs by silver staining. The findings of the present study indicate a greater number of major rDNA clusters in Perna viridis than in closely related species. This is the first comprehensive molecular cytogenetic study carried out in Perna viridis.
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