Development of a Scalable Method for the Isolation and Purification of Recombinant Secretory Phospholipase A2 Expressed in Methylotrophic Yeast Pichia pastoris
2019
Filkin, S. Yu | Chertova, N. V. | Zenin, A. A. | Lipkin, A. V. | Sadykhov, E. G. | Fedorov, A. N.
An efficient recombinant strain of the methylotrophic yeast Pichia pastoris was obtained; it produces active phospholipase A2 (PLA2) in a high yield. As a result of three stages of purification, a preparation of ~90% purity was obtained. The results of the evaluation of the reproducibility of chromatographic separation of PLA2 give grounds to consider the developed protocol acceptable for scaling; isolated phospholipase A2 can be used for industrial applications.
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