Application of sulfur-doped graphene quantum dots@gold-carbon nanosphere for electrical pulse-induced impedimetric detection of glioma cells
2021
Ganganboina, Akhilesh Babu | Dega, Naresh Kumar | Trần, Hải Linh | Darmonto, Win | Doong, Ruey-an
Glioma is the predominant brain tumor with high death rate. The successful development of biosensor to achieve an efficient detection of glioma cells at low concentration remains a great challenge for the personalized glioma therapy. Herein, an ultrasensitive pulse induced electrochemically impedimetric biosensor for glioma cells detection has been successfully fabricated. The 4–11 nm sulfur-doped graphene quantum dots (S-GQDs) are homogeneously deposited onto gold nanoparticles decorated carbon nanospheres (Au–CNS) by Au-thiol linkage to form S-GQDs@Au–CNS nanocomposite which acts as dual functional probe for enhancing the electrochemical activity as well as conjugating the angiopep-2 (Ang-2) for glioma cell detection. Moreover, the application of an externally electrical pulse at +0.6 V expend the surface of glioma cells to accelerate the attachment of glioma cells onto the Ang-2-conjugated S-GQDs@Au–CNS nanocomposite, resulting in the enhanced sensitivity toward glioma cell detection. An ultrasensitive impedimetric detection of glioma cells with a wide linear range of 100–100,000 cells mL⁻¹ and a limit of detection of 40 cells mL⁻¹ is observed. Moreover, the superior selectivity with long-term stability of the developed biosensor in human serum matrix corroborates the feasibility of using S-GQDs@Au–CNS based nanomaterials as the promising sensing probe for practical application to facilitate the ultrasensitive and highly selective detection of cancer cells.
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