Rhizobium halotolerans sp. nov., Isolated from Chloroethylenes Contaminated Soil
2013
Diange, Eboa Adolf | Lee, Sang-Seob
The strain designated as AB21ᵀ was isolated from chloroethylenes contaminated soil. Cells are gram-negative, aerobic, non-spore-forming, and motile rods. Phylogenetic analysis based on 16S rRNA gene sequence showed that it belonged to the genus Rhizobium, and was closely related to Rhizobium sullae IS 123ᵀ (97.4 %), Rhizobium yanglingense SH 22623ᵀ (97.2 %), Rhizobium gallicum R 602spᵀ (97.1 %), Rhizobium alamii GBV 016ᵀ (97.0 %), and Rhizobium monogolense USDA 1844ᵀ (97.0 %). It showed less than 97 % identity with the remaining Rhizobium species. This novel isolate grew optimally at 25–37 °C (optimum, 30 °C) and pH 6–9 (optimum, pH 8.0). It grew in the presence of 0–4 % (w/v) NaCl, tolerating a 4 % (w/v) NaCl. DNA–DNA hybridization experiment shows less than 53 % binding with closely related Rhizobium. Predominant quinone is ubiquinone (Q-10). The major fatty acids were summed feature 8 (composed of C₁₈:₁ ω7c/C₁₈:₁ ω6c), C₁₉:₀ cyclo ω8c, and C₁₆:₀. The G+C molar content is 62.5 mol%. Based on the polyphasic analysis, strain AB21ᵀ is referred to be a novel species of the genus Rhizobium for which the name Rhizobium halotolerans sp. nov. is proposed. The type strain is AB21ᵀ (=KEMC 224-056ᵀ = JCM 17536ᵀ).
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