Chicken interleukinâ6: cDNA structure and biological properties
2001
Schneider, Kirsten | Klaas, Reinhard | Kaspers, Bernd | Staeheli, Peter
Suppression subtractive hybridization technology was used to identify differentially expressed genes in spleens of chickens that had been treated with the synthetic immune modifier Sâ28463. One induced chicken gene encoded a protein with about 35% sequence identity to human interleukinâ6 (ILâ6). It consists of 241 amino acids including a putative Nâterminal signal peptide of 47 residues. Bacterially expressed chicken ILâ6 (ChILâ6) carrying a histidine tag in place of the signal peptide was biologically active: it induced proliferation of the ILâ6âdependent murine hybridoma cell line 7TD1. The concentration of ChILâ6 required for halfâmaximal proliferative response was ≈â60âpg·mL−1. When injected intravenously into adult chickens, purified recombinant ChILâ6 induced an increase in serum corticosterone levels. Supernatants of chicken LMH and monkey COSâ7 cells transiently transfected with a ChILâ6 expression construct induced proliferation of 7TD1 cells, demonstrating that recombinant ChILâ6 from eukaryotic cells is also active.
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