Structural characteristics and enhanced biological activities of partially degraded arabinogalactan from larch sawdust
2021
Cheng, Junwen | Wei, Chaoyang | Li, Weiqi | Wang, Yanbin | Wang, Shihao | Huang, Qingrong | Liu, Yu | He, Liang
Larch arabinogalactan (AG), extracted from Larix gmelinii sawdust, was depolymerized by H₂O₂ oxidation and purified by gel column to yield a novel degraded fraction (AGD2). The structural analysis indicated AGD2 had lower arabinose content and molecular weight compared with AG, in which the ratio of galactose and arabinose was changed from 7:3 to 16:1, the molecular weight was decreased from 50.2 kDa to 3.7 kDa, and the chain conformation spread from highly branched structure to flexible strand. It was one kind of β-D-(1 → 3)-galactan with fewer β-D-(1 → 6)-Galp side branches at O-6 position. Further, the results of the Gal-3 binding and immunomodulatory assay suggested that the unbinding force of AGD2 onto Gal-3 was as twice as AG to be 76 ± 11 pN at the loading rate of 0.15 μm/s. It could better promote the secretion of pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) than AG in a dose-dependent manner.
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