Cytogenetic Study of Aegopodium podagraria (Umbelliferae) for Use in Breeding
2020
Romanov, D. V. | Shirnin, S. Yu | Karlov, G. I. | Divashuk, M. G.
Ground elder (Aegopodium podagraria L.) has a number of economically important properties (as food, an excellent honey plant, and a valuable fodder) and also exhibits antirheumatic, diuretic, nervine, and wound-healing effects. A. podagraria plants are used for cytogenetic studies. To gather a collection of A. podagraria plants, an expedition into different regions of Russia was carried out. In the expedition, A. podagraria was not found in places affected by frequent droughts and where the upper layer of soil tends to dry out. Cytologic slides with A. podagraria metaphase chromosomes were prepared by a method of cell spreading modified by the authors. The method combines the advantages of other methods while surpassing them in terms of the quality and number of metaphases in slides. The chromosome counting shows that collected ground elder specimens have 42 chromosomes. No considerable differences in karyotypes of A. podagraria specimens collected in different regions were observed. The observed differences are within the variability of metaphase plates of a single plant. For more accurate identification of chromosomes and their isolated sectors, which is needed to assist in the selection process, the work on developing cytogenetic markers of A. podagraria must be continued.
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