Platinum Nanoparticle-Based Microreactors as Support for Neuroblastoma Cells
2017
Armada-Moreira, Adam | Taipaleenmäki, Essi | Baekgaard-Laursen, Marie | Schattling, Philipp Sebastian | Sebastião, Ana M. | Vaz, Sandra H. | Stadler, Brigitte
Excitotoxicity is a common phenomenon in several neurological diseases, associated with an impaired clearance of synaptically released glutamate, which leads to an overactivation of postsynaptic glutamate receptors. This will, in turn, start an intracellular cascade of neurotoxic events, which include exacerbated production of reactive oxygen species and ammonia toxicity. We report the assembly of microreactors equipped with platinum nanoparticles as artificial enzymes and polymer terminating layers including poly(dopamine). The biological response to these microreactors is assessed in human neuroblastoma cell culture. The microreactors’ function to deplete hydrogen peroxide (H₂O₂) and ammonia is confirmed. While the proliferation of the cells depends on the number of microreactors present, no inherent toxicity is found. Furthermore, the microreactors are able to ameliorate the effects of excitotoxicity in cell culture by scavenging H₂O₂ and ammonia, thus having the potential to provide a therapeutic approach for several neurological diseases in which excitotoxicity is observed.
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