Effects of water-stress on proline content and ornithine aminotransferase activity of radish cotyledons
1994
Hervieu, F. | Le Dily, F. | Billard, J.P. | Huault, C.
The concentration of proline and the level of ornithine aminotransferase (OAT) activity in light-grown cotyledons of radish (Raphanus sativus cv National) are increased as an inverse function of the relative water content. Gabaculine, which is an irreversible inhibitor of plant OAT, reduced the water-stress induced proline accumulation considerably. It can be assumed that the ornithine pathway contributes via an increase of OAT activity, to proline synthesis, in addition to the glutamate pathway, in water-stressed cotyledons. Drought-induced OAT activity appears to be due to an increase in one of the two OAT isoforms which have been separated by anion-exchange chromatography.
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