Ethanol production from corn cob pretreated by the ammonia steeping process using genetically engineered yeast
1996
Cao, N.J. | Krishnan, M.S. | Du, J.X. | Gong, C.S. | Ho, N.W.Y. | Chen, Z.D. | Tsao, G.T.
A new and effective pretreatment process for biomass conversion involves the steeping of biomass in 2.9 M NH4OH. This resulted in the removing about 80-90% of the lignin along with almost all the acetate from cellulosic residues. Based on dry cellulose from corn cob, a high glucose yield of 92% was obtained after enzymatic saccharification of cellulose fraction. By using a genetically engineered, xylose-fermenting Saccharomyces 1400(pLNH33) in the batch fermentation of a glucose-xylose mixture from corn cob, an ethanol concentration of 47 g/L was obtained within 36 h with 84% yield. In addition, an ethanol concentration of 45 g/L was obtained within 48 h with 86% yield using simultaneous saccharification-fermentation process.
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