Test methods for estimating the efficacy of the fast‐acting disinfectant peracetic acid on surfaces of personal protective equipment
2017
Lemmer, K. | Howaldt, S. | Heinrich, R. | Röder, A. | Pauli, G. | Dorner, B.G. | Pauly, D. | Mielke, M. | Schwebke, I. | Grunow, R.
AIMS: The work aimed at developing and evaluating practically relevant methods for testing of disinfectants on contaminated personal protective equipment (PPE). METHODS AND RESULTS: Carriers were prepared from PPE fabrics and contaminated with Bacillus subtilis spores. Peracetic acid (PAA) was applied as a suitable disinfectant. In method 1, the contaminated carrier was submerged in PAA solution; in method 2, the contaminated area was covered with PAA; and in method 3, PAA, preferentially combined with a surfactant, was dispersed as a thin layer. In each method, 0·5–1% PAA reduced the viability of spores by a factor of ≥6 log₁₀ within 3 min. The technique of the most realistic method 3 proved to be effective at low temperatures and also with a high organic load. Vaccinia virus and Adenovirus were inactivated with 0·05–0·1% PAA by up to ≥6 log₁₀ within 1 min. The cytotoxicity of ricin was considerably reduced by 2% PAA within 15 min of exposure. CONCLUSIONS: PAA/detergent mixture enabled to cover hydrophobic PPE surfaces with a thin and yet effective disinfectant layer. SIGNIFICANCE AND IMPACT OF THE STUDY: The test methods are objective tools for estimating the biocidal efficacy of disinfectants on hydrophobic flexible surfaces.
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