The extraction process optimization and physicochemical properties of polysaccharides from the roots of Euphorbia fischeriana
2011
Liu, Jicheng | Sun, Yongxu | Liu, Lei | Yu, Chunlei
Response surface methodology (RSM) was used to determine the optimum extraction conditions for polysaccharides (EFP) from the roots of Euphorbia fischeriana. A Box–Behnken design (BBD) with four independent variables was investigated, such as extraction temperature (°C), water/solid ratio, extraction number (n), and extraction time (h). The results indicated optimum extraction conditions were extraction temperature of 97°C, water/solid ratio of 9:1, extraction number of 2 and extraction time of 2.4h, respectively. Under these conditions, the experimental value was 24.6±0.62, which was well in close agreement with value predicted by the model. The preliminary chemical analysis of EFP revealed the EFP contained 25.43% polysaccharides, 20.42% uronic acids, 2.54% sulfate radical and 23.41% proteins. And the neutral polysaccharides were mainly composed of glucose, arabinose, rhamnose, galactose, xylose, mannose in the ratio of 21:8:5:3:1:1.
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