In vivo binding of mannose specific lectin from garlic to intestinal epithelium
1997
Gupta, A. | Sandhu, R.S.
The present study was undertaken to determine the effect of high molecular weight (110 kDa) garlic (Allium sativum) lectin (ASA110) on body weight changes and its fate in the intestine of rats. Purified lectin or native/heat treated crude garlic extracts were administered to male swiss albino rats by intragastric intubation route for 7 days. The biological effects included arrest of gain in body weight, which was associated with decreased food intake. Results show that as lectin passed through the gut, it specifically bound to various cellular components of the intestinal mucosa. Although, jejunum showed the presence of mannose receptors located in the core of the lamina propria, duodenum showed lectin binding in the basal region and brush border membrane. Similar binding pattern between ASA110 and normal intestinal mucosa was confirmed in vitro on tissue sections using fluorescent technique. Lectin activity was detected in the fecal extract of treated rats and was antigenically similar to the native lectin preparation. It suggests that the garlic lectin could withstand all enzymatic reactions while passing through the gut. However, its binding to the mucosal epithelium of gastrointestinal tract was indicative of deleterious effects.
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