Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine
2011
Konry, Tania | Dominguez-Villar, Margarita | Baecher-Allan, Clare | Hafler, David A. | Yarmush, Martin.L.
Here we present a microfluidic method for the analysis of single cell secretions. The method co-encapsulates cells with microspheres conjugated with capture antibodies and detection fluorescence-labeled antibodies. The secreted substance captured on the microsphere surface and detected via detection antibodies generating a localized fluorescent signal on a microsphere surface. Using this method, CD4+CD25+ regulatory T cells were encapsulated and assayed to detect IL-10 secreting cell in population.
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