Frondihabitans cladoniiphilus sp. nov., an actinobacterium of the family Microbacteriaceae isolated from lichen, and emended description of the genus Frondihabitans
2011
Cardinale, Massimiliano | Grube, Martin | Berg, Gabriele
A novel actinobacterium, designated strain CafT13T, was isolated from the thallus of the reindeer lichen Cladonia arbuscula sampled in the Austrian Alps (Koralpe). The organism was aerobic, with rod- to irregular-shaped cells (often forming dense clusters of cells when grown in liquid medium), Gram-stain-positive, oxidase-negative, catalase-positive and non-motile. It was able to grow at 1 °C and at low to neutral pH, but not above 30 °C or at high pH. The peptidoglycan type was B2β with ornithine as the diagnostic diamino acid. The menaquinones were MK-7 and MK-8. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, three unidentified glycolipids and one unidentified aminolipid. The predominant fatty acids were C18 : 1, C14 : 0 2-OH, C17 : 1Ï9c, C16 : 0 and anteiso-C15 : 0. The mean DNA G+C content of strain CafT13T was 69.0±0.17 mol%. 16S rRNA gene sequence analysis showed that strain CafT13T belongs to the family Microbacteriaceae, within the genus FRONDIHABITANS: The mean level of DNA–DNA relatedness between strain CafT13T and the type strain of Frondihabitans australicus was 35.2±5.23 %. The enzyme spectrum of strain CafT13T differentiated it from recognized species of the genus FRONDIHABITANS: Based on molecular, chemotaxonomic and physiological data, strain CafT13T is considered to represent a novel species of the genus Frondihabitans, for which the name Frondihabitans cladoniiphilus sp. nov. is proposed; the type strain is CafT13T ( = DSM 23273T = LMG 25550T).
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