Cell wall pectin content modulates aluminium sensitivity of Zea mays (L.) cells grown in suspension culture
2000
Schmohl, N. | Horst, W.J.
The pectin content of the cell walls of maize suspension cells was modified to investigate its role in the expression of aluminium (Al) toxicity. Long-term adaptation to NaCl or to the cellulose synthesis inhibitor 2,6-dichlorbenzonitrile (DCB) increased the pectin content by 31 and 86%, respectively. Subculturing salt-adapted cells for up to 3 weeks without NaCl supply or treatment of cells with pectolyase for up to 15 min reduced pectin contents by up to 46%. Such pre-cultured cells were incubated for 2 h in presence of Al. There was a close positive correlation between pectin and both total and BaCl(2) non-exchangeable Al contents. Aluminium-induced callose formation as an indicator of Al injury was closely positively correlated to the loss of cell viability. In NaCl-adapted and pectolyase-treated cells, Al-induced callose formation was reduced when compared with normal cells. However, there was a close positive relationship between pectin contents and relative callose induction (digitonin-induced callose formation, reflecting the different capabilities of cells to synthesize callose set to 100%) indicating that cells with higher pectin contents are more Al-sensitive. The results presented support our view that the binding of Al to the cell wall pectin-matrix represents an important step in the expression of Al toxicity.
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