Variations in endogenous viral gene patterns in White Leghorn, medium heavy, White Plymouth Rock, and Cornish chickens
1991
Aarts, H.J.M. | Hulst-Van Arkel, M.C. van der | Beuving, G. | Leenstra, F.R.
The endogenous viral (ev) gene patterns of White Leghorn (WL; 6 lines), medium heavy (MH; 4 lines), White Plymouth Rock (WPR; 8 lines), and Cornish types (2 lines) of commercial chickens were compared. Southern blot analysis of SstI-digested genomic DNA of 151 chickens revealed that the number of ev gene-containing fragments in a chicken from the MH, WPR, or Cornish type is about twice the number of that in WL chickens. Also, the number of hybridizing fragments of different size found within one line was twice as high in the broiler (on average 16.0 bands per line) and MH lines (20.5 bands per line) than in the WL lines (10.0 bands per line). In studies with subregion-specific probes, all ev fragment detected contained the env (3') part of the viral genome. Only eight ev fragments, found in 7 animals of 2 lines, lacked the gag (5') part of the viral genome. Studies with the ev-1-specific flanking probe, pGd111, revealed that ev-1 is commonly present in the DNA of the WL chickens, but not within the DNA of the WPR chickens. The results suggest that use of the standard nomenclature for the ev genes based on restriction fragment length is not feasible within the WPR, MH and Cornish lines because of the complexity of the ev gene patterns found within these lines.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por National Agricultural Library