Using the 454 pyrosequencing-based technique in the development of nuclear microsatellite loci in the alpine plant Arabis alpina (Brassicaceae)
2011
Buehler, Dominique | Graf, René | Holderegger, Rolf | Gugerli, Felix
Premise of the study: Polymorphic microsatellite markers were developed for the inbred alpine perennial plant Arabis alpina to infer life-history parameters and measure patterns of contemporary gene flow within populations. METHODS: and Results: Using the 454 pyrosequencing technique, 19 microsatellite primer sets were developed for A. alpina. The primer sets were tested on 60 individuals sampled from three sub-populations in the Swiss Alps. The primers amplified di- and trinucleotide repeats with two to five alleles per locus. CONCLUSIONS: Previous attempts to isolate microsatellite loci in A. alpina using enrichment libraries and cross-amplification were difficult and produced an insufficient number of polymorphic microsatellite loci. In contrast, next-generation sequencing technology was successful in identifying microsatellite repeats in A. alpina. These newly developed microsatellite primers will be useful to further develop A. alpina into a model species for eco-genomic studies.
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